BioanalysisVol. 5, No. 10 EditorialHigh-resolution MS: first choice for peptide quantification?Lieve Dillen & Filip CuyckensLieve Dillen* Author for correspondenceDrug Metabolism & Pharmacokinetics, Janssen Research & Development, a Division of Janssen Pharmaceutica N.V., Turnhoutseweg 30, 2340 Beerse, Belgium. Search for more papers by this authorEmail the corresponding author at ldillen@its.jnj.com & Filip CuyckensJanssen Research & Development, a Division of Janssen Pharmaceutica N.V., BelgiumSearch for more papers by this authorPublished Online:30 May 2013https://doi.org/10.4155/bio.13.90AboutSectionsView ArticleView Full TextPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareShare onFacebookTwitterLinkedInReddit View articleKeywords: HRMSorthogonal assaypeptidesquantificationSRMReferences1 Ramigiri S, Garofolo F. Large molecule bioanalysis using Q-TOF without predigestion and its data processing challenges. Bioanalysis4(5),529–540 (2012).Link, Google Scholar2 Dillen L, Cools W, Vereyken L et al. Comparison of triple quadrupole and high resolution time of flight mass spectrometry for quantification of peptides. Bioanalysis4(3),565–579 (2012).Link, CAS, Google Scholar3 Korfmacher W. High resolution mass spectrometry will dramatically change our drug-discovery bioanalysis procedures. Bioanalysis3(11),1169–71 (2011).Link, CAS, Google Scholar4 Ramanathan R, Jemal M, Ramagiri S et al. It is time for a paradigm shift in drug discovery bioanalysis: from SRM to HRMS. J. Mass Spectrom.46,595–601 (2011).Crossref, Medline, CAS, Google Scholar5 Iavarone AT, Jurchen JC, Williams ER. Effects of solvent on the maximum charge state and charge state distribution of protein ions produced by electrospray ionization. J. Am. Soc. Mass. Spectrom.11(11),976–985 (2000).Crossref, Medline, CAS, Google Scholar6 Morin L-P, Mess J-N, Garofolo F. Large-molecule quantification: sensitivity and selectivity head-to-head comparison of triple quadrupole with Q-TOF. Bioanalysis5(10),1181–1193 (2013).Link, CAS, Google Scholar7 Campbell JL, Le Blanc JCY. Targeted ion parking for the quantitation of biotherapeutic proteins: concepts and preliminary data. J. Am. Soc. Mass Spectrom.21(12),2011–2022 (2010).Crossref, Medline, CAS, Google Scholar8 Hao C, Campbell JL, Verkerk UH, Le Blanc JCY, Siu KWM. Performance and attributes of liquid chromatography–mass spectrometry with targeted charge separation in quantitative analysis of therapeutic peptides. J. Am. Soc. Mass Spectrom.22(1),67–74 (2011).Crossref, Medline, CAS, Google Scholar9 Cuyckens F, Dillen L, Cools W et al. Extracting metabolite ions of peptide drugs from an in vivo matrix background. Bioanalysis4(3),595–604 (2012).Link, CAS, Google Scholar10 Timmerman P, Henderson N, Smeraglia J et al. Managing scientific, technical and regulatory innovation in regulated bioanalysis: a discussion paper from the European Bioanalytical Forum. Bioanalysis5(2),139–145 (2013).Link, CAS, Google Scholar11 Rochat B, Kottelat E, McMullen J. The future key role of LC–high-resolution-MS analyses in clinical laboratories: a focus on quantification. Bioanalysis4(24),2939–2958 (2012).Link, CAS, Google Scholar101 2012 EBF Open Symposium. HRMS in regulated bioanalysis? http://bcn2012.europeanbioanalysisforum.eu/slides/day%202/v%20hrms/1_bateman.pdfGoogle ScholarFiguresReferencesRelatedDetailsCited ByA simple, sensitive, high-resolution, customized, reverse phase ultra-high performance liquid chromatographic method for related substances of a therapeutic peptide (bivalirudin trifluoroacetate) using the quality by design approach1 January 2020 | Analytical Methods, Vol. 12, No. 3Microflow UPLC and high-resolution MS as a sensitive and robust platform for quantitation of intact peptide hormonesZhuo Chen, Yun W Alelyunas, Mark D Wrona, Jonathan R Kehler, Matthew E Szapacs & Christopher A Evans12 July 2019 | Bioanalysis, Vol. 11, No. 13Mass spectrometry in drug metabolism and pharmacokinetics: Current trends and future perspectives4 September 2018 | Rapid Communications in Mass Spectrometry, Vol. 33, No. S3A liquid chromatography high-resolution mass spectrometry in vitro assay to assess metabolism at the injection site of subcutaneously administered therapeutic peptidesJournal of Pharmaceutical and Biomedical Analysis, Vol. 159Targeted Quantification of Peptide Biomarkers14 July 2017Automated SPE and nanoLC–MS analysis of somatostatin8 May 2017 | Journal of Liquid Chromatography & Related Technologies, Vol. 40, No. 8An efficient liquid chromatography-high resolution mass spectrometry approach for the optimization of the metabolic stability of therapeutic peptides30 January 2017 | Analytical and Bioanalytical Chemistry, Vol. 409, No. 10HRMS using a Q-Exactive series mass spectrometer for regulated quantitative bioanalysis: how, when, and why to implementRobert M Sturm, Barry R Jones, Daniel E Mulvana & Steve Lowes27 July 2016 | Bioanalysis, Vol. 8, No. 16Toward greener analytical techniques for the absolute quantification of peptides in pharmaceutical and biological samplesJournal of Pharmaceutical and Biomedical Analysis, Vol. 113Liquid Chromatography-High Resolution Mass Spectrometry for Peptide Drug Quality Control26 February 2015 | The AAPS Journal, Vol. 17, No. 3Quantitation of human peptides and proteins via MS: review of analytically validated assaysDerek L Chappell, Michael E Lassman, Thomas McAvoy, Mingxiang Lin, Daniel S Spellman, Omar F Laterza26 August 2014 | Bioanalysis, Vol. 6, No. 13Preface to the 2013 Special Focus Issue of Bioanalysis on high-resolution MSWalter Korfmacher & Ragu Ramanathan30 May 2013 | Bioanalysis, Vol. 5, No. 10 Vol. 5, No. 10 Follow us on social media for the latest updates Metrics Downloaded 429 times History Published online 30 May 2013 Published in print May 2013 Information© Future Science LtdKeywordsHRMSorthogonal assaypeptidesquantificationSRMFinancial & competing interests disclosureThe authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.No writing assistance was utilized in the production of this manuscript.PDF download